RT-qPCR mix for probes without ROX
Mix for quantitative RT-PCR for probes without ROX are made for use with qPCR devices that do not require this internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations.
The mix for probes consists of a Reverse transcriptase, a Taq polymerase, dNTPs, MgCl2. It is possible to add ROX dye to these mixes for use with qPCR devices requiring the presence of ROX.
We offer a probe synthesis service for qPCR
Here's how to select the appropriate ROX dye for the qPCR device you are using:
| Universal | All instruments |
| Without ROX | Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
| Low ROX | Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P |
| High ROX | Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus |
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