SIMOA (Single Molecule Array) technology represents a significant advancement in biomarker detection and quantification. This innovative platform allows for ultra-sensitive measurements of proteins, enabling researchers to detect biomarkers at concentrations that were previously undetectable. SIMOA technology utilizes a digital immunoassay format that can measure protein concentrations in the femtomolar (fg/mL) range, which is more sensitive than traditional ELISA methods. This capability is particularly valuable in clinical research and diagnostics, where low-abundance biomarkers can provide critical insights into disease mechanisms and therapeutic responses. The technology works by isolating individual immuno-complexes in femtoliter-sized reaction chambers, allowing for the precise counting of molecules rather than relying on bulk measurements.
Key Features
- Ultra-Sensitivity: SIMOA can detect proteins at extremely low concentrations, making it ideal for applications in areas such as oncology, neurology, and cardiology.
- Multiplexing Capability: The platform allows for the simultaneous measurement of multiple biomarkers from a single sample, enhancing efficiency and reducing sample volume requirements.
- High Throughput: SIMOA technology supports high-throughput workflows, enabling researchers to process large numbers of samples quickly and accurately.
Available Assays
We are currently offering the below ultrasensitive human assays for immediate testing:
| Amyloid beta 1-40 | GM-CSF | IL-12 p70 | IL-17A | IL-6 | MCP-1 (CCL2) | TNF alpha |
| C-Peptide | IFN-gamma | IL-13 | IL-2 | IL-7 | p24 | TRAIL (TNFSF10) |
| Eotaxin-1 (CCL11) | IL-1 beta (IL-1 F2) | IL-15 | IL-23 | IL-8 (CXCL8) | PSA | Troponin I |
| GCSF | IL-10 | IL-16 | IL-5 | IP-10 (CXCL10) | Tau |
Don't see the biomarker you're looking for? The entire immunoassay menu (>1,600 targets covering 12 different species) is available for conversion to the SIMOA testing service platform. Simply request the biomarker you are interested in and we will develop the SIMOA for it. Please note there is an additional 4-6 week lead time.
How SIMOA Works
SIMOA is an ultrasensitive ELISA that uses antibody-coated beads, a fluorescently conjugated detection antibody, and digital readout. After the beads, sample, and detection antibody have been mixed together, the solution is applied to a cartridge with over 235,000 microwells. Each microwell can hold only one bead. Oil is then added to the cartridge, which pushes the sample across the surface of the microwell array and forms a liquid-tight seal of the wells.
The fluorescence of each well is measured using a fully or semi-automated system equipped with a fluorescent microscope. The instrument is manufactured by the developer of SIMOA (Quanterix; Billerica, MA; USA). Quantitation of target protein concentration is based on the number of microwells with fluorescent signal and not the intensity of the fluorescent signal, making the readout a "digital" one. Since each well can contain only one bead, the number of microwells with fluorescence is proportional to the amount of target protein in the sample. This approach enables quantitative, ultrasensitive single molecule detection.
