Plasmid DNA Purification: The Efficiency of EasyPure® 1-Tube Universal EndoFree MaxiPrep Kit
Optimize plasmid DNA extraction with EasyPure® 1-Tube MaxiPrep Kit—fast, efficient, high-yield, and ideal for seamless downstream applications.
EasyPure® 1-Tube Universal EndoFree Plasmid MaxiPrep Kit (for 100-500 mL) uses a modified lysis system to lyse E. coli cells to efficiently release plasmid DNA, followed by the binding of the DNA onto the silica membrane spin column. Lysis and neutralization of 100-500 mL E. coli liquid cultured in Lysogeny broth (LB) medium can be completed in a single centrifuge tube; the volume of the supernatant is less than 50 mL. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color so as to visualize the operation. The purified DNA is suitable for restriction enzyme digestion, ligation, transformation, sequencing, transfection, etc.
Features
1-tube type: Lysis and neutralization of 100-500 mL bacterial liquid can be completed in a single 50 mL centrifuge tube, without the need to prepare large-volume consumables.
Visualization: Solution LB II (blue) indicates whether lysis and neutralization are complete through the change of color, ensuring the quality of plasmid extraction.
Fast: The universal system is compatible with the extraction of 100-500 mL of bacterial liquid. A small-volume system combined with a large-volume spin column greatly reduces the number of centrifugations and saves operation time.
High yield: Up to 5 mg nucleic acid load in the purification column.
Data
Easy to operate and fast: Only 3 centrifugations are required and the extraction can be completed in 80 minutes.
Efficient extraction of high and low copy plasmids from different culture volumes.
Different types of plasmids from different volumes of bacterial culture were extracted using reagent from TransGen. The extracted plasmids were analyzed by concentration, A260/280, A260/230, and agarose gel electrophoresis. The results showed that TransGen product can efficiently extract bacterial cultures of different volumes, with high quality.
Comparison with competitive products
Plasmids from different volumes of bacterial culture were extracted using reagents from TransGen, Company T and Company M. The extracted plasmids were analyzed by concentration, A260/280, A260/230, and agarose gel electrophoresis. The results show that plasmid extracted with TransGen product has higher quality.
Downstream application - cell transfection
GFP plasmids were extracted using regaents from TransGen and Company M. 293T cells were transfected with plasmids of equal quality, respectively. Transfection results were detected with fluorescence microscopy. The results showed that plasmid extracted with TransGen product can be successfully used for cell transfection experiment, with high transfection efficiency.
Explore how the EasyPure® 1-Tube Universal EndoFree Plasmid MaxiPrep Kit enhances efficiency in plasmid DNA preparation for downstream applications such as cloning, sequencing, and transfection.
