Gene synthesis
We propose to produce a double-stranded DNA from your nucleotidic sequence.
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This DNA can be shipped in a fragment form or cloned in a plasmid.
We also offer you the opportunity to modify your codons in order to optimize your recombinant production yield.
With this DNA, you will be able to realize :
- Protein production
- Cell transfection
- Genetic immunization
- Gene therapy
Prestation 1 : Standard protocol for the DNA fragment obtention :
- Double-stranded DNA synthesis
- Option : Re-synthesis to obtain more material
Prestation 2 : Checking of synthetized DNA :
- Double-stranded DNA synthesis
- Sequencing
- Mutagenesis run in order to remove errors
Key numbers :
- Synthesis efficiency : 99,9%(an average 1 error every 1000 bp)
- Final efficiency : 100%if errors have been removed
- Quantity : For prestation 1, enough to perform 10 cloning reactions
- Delay : Prestation 1 : 15 days / Prestation 2 : cloned and sequenced, according to fragment length (3-8 weeks)
Related services available :
=> Cloning
=> Polyclonal antibody production
=> Monoclonal antibody production