Trastuzumab (Herceptin) Immunogenicity ELISA
Cat# EL-141-201
Size : 1X96wells
Brand : AffinityImmuno
This Trastuzumab (Herceptin®) Immunogenicity ELISA kit allows researchers to qualitatively monitor the presence of anti-Trastuzumab antibodies in biological matrices. Trastuzumab (Herceptin® ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2).
INTRODUCTION
Trastuzumab (Herceptin® ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2). Repeated dosing of Trastuzumab has been reported to induce a neutralizing antibody response resulting in reduce efficacy of Trastuzumab. This kit allows
researchers to qualitatively monitor the presence of anti-Trastuzumab antibodies in biological matrices.
PRINCIPLE OF THE ASSAY
This immunogenicity assay employs the bridging ELISA technique. Capture antibody is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti- Trastuzumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development.
The color development is proportional to the amount of anti-Trastuzumab present in test samples. Three levels of QC samples give a qualitative reference signal which can be used to determine the level of anti-Trastuzumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.
-
Catalog Number:
EL-141-201 -
Application(s)
measure anti-trastuzumab antibodies -
Target Protein
herceptin -
Alternative Name of Target
trastuzumab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
ntra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
31.3ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells QC samples - 4x50ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X secondary antibody - 17ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Product Specifications
-
Catalog Number:
EL-141-201 -
Application(s)
measure anti-trastuzumab antibodies -
Target Protein
herceptin -
Alternative Name of Target
trastuzumab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
ntra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
31.3ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells QC samples - 4x50ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X secondary antibody - 17ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3