Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western blot using TMBIM1 polyclonal antibody (Cat # PAB11278) shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-TMBIM1 (Lane 1).
No staining is observed in lysates from mock transformed HeLa cells (Lane 2).
To date this antibody has shown the ability to recognize overexpressed TMBIM1, but not endogenous protein.
The primary antibody at a 1:1,000 dilution.
Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against partial recombinant TMBIM1.

    Immunogen

    Recombinant protein corresponding to N-terminus of human TMBIM1.

    Host

    Rabbit

    Reactivity

    Human, Mouse, Rat

    Form

    Liquid

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Recommend Usage

    ELISA (1:10000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Transfected lysate)

    Western blot using TMBIM1 polyclonal antibody (Cat # PAB11278) shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-TMBIM1 (Lane 1).
    No staining is observed in lysates from mock transformed HeLa cells (Lane 2).
    To date this antibody has shown the ability to recognize overexpressed TMBIM1, but not endogenous protein.
    The primary antibody at a 1:1,000 dilution.
    Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — TMBIM1

    Entrez GeneID

    64114

    Protein Accession#

    NP_071435;Q969X1

    Gene Name

    TMBIM1

    Gene Alias

    MST100, MSTP100, PP1201, RECS1

    Gene Description

    transmembrane BAX inhibitor motif containing 1

    Omim ID

    610364

    Gene Ontology

    Hyperlink

    Other Designations

    -

  • Interactomes