Propidium Iodide [25535-16-4]
Cat# T2130-25mg
Size : 25mg
Brand : TargetMol
Propidium Iodide
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Purity:98.88%
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COA HPLC HNMR
Propidium Iodide
Catalog No. T2130Cas No. 25535-16-4
Propidium Iodide (PI) is a red fluorescent dye that can be used for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of Propidium Iodide is 493/636 nm. And after binding with nucleic acid, the Ex/Em of PI-nucleic acid becomes 535/617 nm, while the fluorescence signal is enhanced 20-30 times.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose. Pack Size | |
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10 mg | |
25 mg | |
50 mg | |
100 mg |
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
Description | Propidium Iodide (PI) is a red fluorescent dye that can be used for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of Propidium Iodide is 493/636 nm. And after binding with nucleic acid, the Ex/Em of PI-nucleic acid becomes 535/617 nm, while the fluorescence signal is enhanced 20-30 times. |
In vitro | METHODS: Propidium Iodide uptake and Flow cytometry to detect cell death: 1. Store the PI stock solution (0.5 mg/mL in PBS) in a dark place at 4℃. Immediately before use, prepare PI-FACS buffer by adding 20 µL of PI stock solution per 1 mL of PBS. 2. Collect suspended cells: Collect cells directly in centrifuge tubes and centrifuge at 500g for 5 min to harvest all cells. 3. Collect adherent cells: Remove and preserve the medium containing dead and mitotic cells. Isolate live cells using standard tissue culture techniques, such as incubation with trypsin-EDTA, and be sure to collect any washings (e.g., PBS). Add cells from the culture medium and cells from any wash solution to the isolated cells and centrifuge at 500 g for 5 min to harvest all cells. 4. Resuspend the harvested cells in PI-FACS buffer. The cells were incubated in the dark for 15 min at room temperature. 5. Determine the cell mortality rate by flow cytometry. [1] |
Alias | PI |
Molecular Weight | 668.39 |
Formula | C27H34I2N4 |
Cas No. | 25535-16-4 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | |||||||||||||||||||||||||
Solubility Information | H2O: 5 mg/mL (7.48 mM), Sonication and heating to 60℃ are recommended. DMSO: 6.68 mg/mL (10 mM), Sonication is recommended. | |||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||
H2O/DMSO
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSO
main solution, add 300 μLPEG300
mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O
mix well and clarify
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
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