Pax2 (Paired Box Gene 2, Paired Box Homeotic Gene 2, Paired Box Protein 2, Paired Box Protein Pax-2, Paired Box Protein Pax2, Pax-2) (Biotin)

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. The paired box containing genes were first identified in Drosophila and were subsequently described in a variety of species including mouse, human, chicken, Xenopus and Zebrafish. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6 is associated with Aniridia, and pax-2 which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms’ tumors. Persistent expression of Pax-2 in Wilms’ tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms’ tumor suppresser protein WT1 coincides with down-regulation of the pax-2 gene, and the WT1 protein can directly repress pax-2 transcription. Therefore, pax-2 is thought to be a target gene for WT1. ||Applications: |Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry and Immunofluorescence. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.