WESTAR ANTARES is an ECL substrate with stable light output for mid femtogram detection level.
The perfect ECL formulation combining great sensitivity and long signal duration.
NeoPRO Pico PLUS Western ECL Substrate
Cat# NB-78-00003
Size : 2x125mLkit
Brand : Neo Biotech
Properties for WESTAR ANTARES
| Description | WESTAR ANTARES is an ECL substrate with stable light output for mid femtogram detection level. The perfect ECL formulation combining great sensitivity and long signal duration. |
| Features | Mid femtogram detection Extended signal duration Working solution stable for at least three days High range flexibility |
| Components | Luminol/enhancer solution (A) Peroxide solution (B) |
| Antibody Dilution Range | Primary: 1:1.000 - 1:15.000 Secondary: 1:25.000 - 1:150.000 (from 1 mg/mL stock solution) |
| Available as | 2 x 125 mL kit (2500 cm2 of membrane) |
| Signal duration | > 24 hours |
| Storage | Stable for 1 year at RT Product is shipped at ambient temperature |
Online Resources & Supporting Documents
Gallery
Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR ANTARES and its competitors.
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:5000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:75000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:5000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:75000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Extended signal duration of WESTAR ANTARES.
For either WESTAR ANTARES or its competitors, exposure time is 180 seconds for each time points (0-2-5-8-11-20 hours).
For either WESTAR ANTARES or its competitors, exposure time is 180 seconds for each time points (0-2-5-8-11-20 hours).