WESTAR SUPERNOVA is a high performance product with an exceptional signal intensity and stable light output for low femtogram detection level.
The formulation provides a low background for a high signal to noise ratio.
NeoPRO Femto PLUS Western ECL Substrate
Cat# NB-78-00005
Size : 2x50mLkit
Brand : Neo Biotech
Properties for WESTAR SUPERNOVA
| Description | WESTAR SUPERNOVA is a high performance product with an exceptional signal intensity and stable light output for low femtogram detection level. The formulation provides a low background for a high signal to noise ratio. |
| Features | Low femtogram detection Signal intensity equals to current top-level competitors at least Working solution stable for at least three days Low antibody consumption to save your money |
| Components | Luminol/enhancer solution (A) Peroxide solution (B) |
| Antibody Dilution Range | Primary: 1:5.000 - 1:100.000 Secondary: 1:100.000 - 1:500.000 (from 1 mg/mL stock solution) |
| Available as | 2 x 50 mL kit (1000 cm2 of membrane) |
| Signal duration | 11 hours |
| Storage | Stable for 1 year at RT Product is shipped at ambient temperature |
Online Resources & Supporting Documents
Gallery
Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR SUPERNOVA and its competitors.
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 2.5 μg to 0,039 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:15000
Secondary antibody: Goat anti-Rabbit IgG HRP (2mg/mL) (abcam®) 1:300000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 120 seconds
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 2.5 μg to 0,039 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:15000
Secondary antibody: Goat anti-Rabbit IgG HRP (2mg/mL) (abcam®) 1:300000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 120 seconds
WESTAR SUPERNOVA Signal Duration Comparison with Competitors
Comparison of signal intensities at time points up to 20 hours post substrate addition.
Exposure time is 120 seconds for each time point (0-1-2-5-8-11 hours).
Comparison of signal intensities at time points up to 20 hours post substrate addition.
Exposure time is 120 seconds for each time point (0-1-2-5-8-11 hours).