Mouse Monoclonal Anti-Plakophilin 1, supernatant [Clone PP1-5C2]
Cat# 65160
Size : 5mL
Brand : Progen
Quantity | 5 ml |
---|---|
Antibody Type | Monoclonal |
Host | Mouse |
Isotype | IgG1 |
Conjugate | Unconjugated |
Application | ELISA, IHC, WB |
Purification | Hybridoma cell culture supernatant |
Reactivity | Bovine, Human, Mouse, Pig, Rat |
Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Clone | PP1-5C2 |
Immunogen | Plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis) |
Formulation | Contains 0.09% sodium azide |
UniprotID | Q28161 (Bovine), Q13835 (Human), P97350 (Mouse), I3LGN8 (Pig), D3ZY51 (Rat) |
Synonym | Plakophilin-1, Band 6 protein, B6P, PKP1 |
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | Undiluted-1:50 (preincubation with 0.05-0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred) |
Immunohistochemistry (IHC) - paraffin | Undiluted-1:50 (pretreatment with 0.001% trypsin and microwave treatment recommended) |
ELISA | Assay dependent |
Western Blot (WB) | Assay dependent |
Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.
Polypeptide reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine).
Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue).
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