MLi-2 [1627091-47-7]
Cat# HY-100411-5mg
Size : 5mg
Brand : MedChemExpress
MLi-2 est un inhibiteur oralement active et hautement sélectif de LRRK2 avec un IC50 de 0,76 nM. MLi-2 a le potentiel pour la maladie de Parkinson.
MLi-2 ist ein oral aktiver und hochselektiver LRRK2-Inhibitor mit einem IC50 von 0,76 nM. MLi-2 hat das Potenzial für die Parkinson-Krankheit.
MLi-2 is an orally active and highly selective LRRK2 inhibitor with an IC50 of 0.76 nM. MLi-2 has the potential for Parkinson’s disease.
For research use only. We do not sell to patients.
MLi-2 Chemical Structure
CAS No. : 1627091-47-7
This product is a controlled substance and not for sale in your territory.
Based on 4 publication(s) in Google Scholar
Description |
MLi-2 is an orally active and highly selective LRRK2 inhibitor with an IC50 of 0.76 nM. MLi-2 has the potential for Parkinson’s disease[1]. |
IC50 & Target |
IC50: 0.76 nM (LRRK2)[1] |
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In Vitro |
MLi-2 exhibits exceptional potency in a purified LRRK2 kinase assay in vitro( IC50=0.76 nM), a cellular assay monitoring dephosphorylation of LRRK2 pSer935 LRRK2 (IC50=1.4 nM), and a radioligand competition binding assay (IC50=3.4 nM). MLi-2 has greater than 295-fold selectivity for over 300 kinases in addition to a diverse panel of receptors and ion channels[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Acute oral and subchronic dosing in MLi-2 mice results in dose-dependent central and peripheral target inhibition over a 24-hour period as measured by dephosphorylation of pSer935 LRRK2. Treatment of MitoPark mice with MLi-2 is well tolerated over a 15-week period at brain and plasma exposures. Morphologic changes in the lung, consistent with enlarged type II pneumocytes, are observed in MLi-2-treated MitoPark mice[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
379.46 |
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Formula |
C21H25N5O2 |
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CAS No. |
1627091-47-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
C[C@@](C1)(00)O[C@](C)(00)CN1C2=CC(C3=NNC4=CC=C(OC5(C)CC5)C=C43)=NC=N2 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : 50 mg/mL (131.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.80% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.6353 mL | 13.1766 mL | 26.3532 mL | 65.8831 mL |
5 mM | 0.5271 mL | 2.6353 mL | 5.2706 mL | 13.1766 mL | |
10 mM | 0.2635 mL | 1.3177 mL | 2.6353 mL | 6.5883 mL | |
15 mM | 0.1757 mL | 0.8784 mL | 1.7569 mL | 4.3922 mL | |
20 mM | 0.1318 mL | 0.6588 mL | 1.3177 mL | 3.2942 mL | |
25 mM | 0.1054 mL | 0.5271 mL | 1.0541 mL | 2.6353 mL | |
30 mM | 0.0878 mL | 0.4392 mL | 0.8784 mL | 2.1961 mL | |
40 mM | 0.0659 mL | 0.3294 mL | 0.6588 mL | 1.6471 mL | |
50 mM | 0.0527 mL | 0.2635 mL | 0.5271 mL | 1.3177 mL | |
60 mM | 0.0439 mL | 0.2196 mL | 0.4392 mL | 1.0981 mL | |
80 mM | 0.0329 mL | 0.1647 mL | 0.3294 mL | 0.8235 mL | |
100 mM | 0.0264 mL | 0.1318 mL | 0.2635 mL | 0.6588 mL |