Reagents and instruments for immunology, cell biology and molecular biology.
 
> Lactobacilli MRS Broth, Defined, w/o Thymine, Folic Acid (Powder)

Lactobacilli MRS Broth, Defined, w/o Thymine, Folic Acid (Powder)

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Cat# L1021-07-25L

Size : 25L

Brand : US Biological

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L1021-07 Lactobacilli MRS Broth, Defined, w/o Thymine, Folic Acid (Powder)

Clone Type
Polyclonal
Grade
Microbiological Grade
Shipping Temp
RT
Storage Temp
4°C

This is a special chemically-defined medium without Thymine, Thymidine or Folic Acid.||Suitable for use in the cultivation of Lactobacilli from dairy products and other materials. Lactobacilli MRS Broth is based on the formulations of deMan, Rogosa and Sharpe (MRS). This medium supports luxuriant growth of Lactobacilli from oral, fecal, dairy and other sources.||Animal tissue digest, beef extract and yeast extract, normally the carbon, nitrogen and vitamin sources used to satisfy general growth requirements in Lactobacilli MRS Broth, are absent and replaced with supplements and vitamins. ||Dextrose is the fermentable carbohydrate. Potassium acetate and ammonium citrate act as selective agents as well as energy sources. Potassium phosphate is the buffering agent. Magnesium sulfate and manganese sulfate provide cations used in metabolism. Polysorbate (Tween) 80 is a surfacant, facilitating uptake of nutrients by Lactobacilli.||Appearance:|White to light tan, homogeneous,, free flowing powder.||Solubility: |Yellow to golden yellow, clear after sterile filtering||pH: |As Reported

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. J. Applied Micro., 88:695-703 (2000) 2. deMan, J. C., M. Rogosa and M. E. Sharpe, 1960. A medium for the cultivation of lactobacilli. J. Bacteriol. 23:130. 3. MacFaddin, J. F. 1985. Media for the isolation, cultivation, identification and maintenance of medical bacteria, vol. 1 Williams & Wilkins, Baltimore, MD. 4. Vanderzant, C. and D. F. Splittstoesser (eds.). Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D. C. 5. Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover and R. H. Yolken (eds.). 1995. Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D. C.