Size : 100ml
Request more information
Please log in to use this feature.
JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
HyperChrom Ni-NTA FF Agarose is an affinity chromatography medium made by chemically coupling the transition metal ion Ni2+ with tetracoordination to a ligand of Nitrilotriacetic acid (NTA) using a highly crosslinked agarose gel as a matrix. It has a very stable octahedral structure, nickel ions are at the center of the octahedron, which protects nickel ions from attack by small molecules, is more stable, and can withstand harsh conditions such as reducing agents, denaturants or couplants in a certain concentration. In addition, due to the pressure resistance of the matrix, which can withstand pressures up to 0.3 MPa, the product can be used for industrial large-scale protein purification, enabling purification of the protein of interest at relatively high flow rates. This chromatography medium uses the interaction between Ni2+ and the side chains of certain amino acids (mainly histidine) on the protein to achieve the separation and purification of proteins containing and not containing these amino acids and containing a large and low number of these amino acids.
HyperChrom Ni-NTA FF Agarose is the first choice for purifying histidine-tagged proteins, with the advantages of high load, easy regeneration, and low cost.
HyperChrom Ni-NTA FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
NTA-Ni2+
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ligand density
~15 μmol Ni2+/mL chromatography medium
Dynamic load
≥40 mg histidine-tagged protein/mL chromatography medium*
Flow rates are recommended
90-300 cm/h
Maximum flow rate
400 cm/h
Withstand pressure
0.3 MPa
Use temperature
4 - 30℃
pH stability **
2-14
Chemical resistance
Common aqueous solution, 0.01 M HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol***
* Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 150 cm/h; Test buffer: 0.02 M PB, 0.5 M NaCl, 5 mM imidazole, pH 7.4; test sample: tagged protein (1 mg/ml, 6*His); Index: When the protein penetration reaches 10%, the amount of protein loaded per unit volume of media (mL) (mg).
**After 7 days of storage of chromatography medium at 40 °C and pH2-14 environment, its physicochemical properties and functions did not change significantly.
*** 30% is v/v, volume ratio.
Store the components at 4℃ for 5 years.
