Human Gingival Fibroblasts (hGF)
Cat# 300703
Size : 1cryovial
Brand : CLS Cell Lines Service
Human Gingival Fibroblasts (hGF)
General information
Description | Human gingival fibroblasts (hGFs) are undifferentiated cells with multi-differentiation and self-renewal capacities. These mesenchymal cells provide many vital functions during development and adulthood. Thus, for instance they are responsible for a large part of the synthesis of the extracellular matrix in connective tissue and play an important role in wound healing. |
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Organism | Human |
Tissue | Gingiva |
Applications | Tissue regeneration, Wound healing studies |
Characteristics
Cell type | Fibroblast |
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Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Human Gingival Fibroblasts (hGF) (Cytion catalog number 300703) |
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Expression / Mutation
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS, 10 ng/mL bFGF, 10 microgram/L Insulin |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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