HK-ZFN-AURKB-mEGFP genomic DNA - 5 microgram
Cat# 300173GD5
Size : 5ug
Brand : CLS Cell Lines Service
HK-ZFN-AURKB-mEGFP Cells
General information
Description | The HK-ZFN-AURKB-mEGFP cell line is a genetically engineered human cell model designed to express the AURKB (Aurora Kinase B) protein fused with mEGFP (monomeric Enhanced Green Fluorescent Protein) using Zinc Finger Nuclease (ZFN) technology. AURKB is a serine/threonine kinase that plays a crucial role in mitotic chromosome segregation, cytokinesis, and the regulation of the mitotic spindle checkpoint. The fusion with mEGFP allows for real-time visualization of AURKB activity and localization within the cell, facilitating detailed studies of its dynamic behavior during cell division. This cell line serves as a powerful tool for researchers investigating the molecular mechanisms of mitosis and the specific functions of AURKB. The incorporation of mEGFP enables fluorescence-based assays and live-cell imaging, providing insights into the spatiotemporal distribution of AURKB. The use of ZFN technology ensures precise genomic integration, maintaining the fidelity of AURKB expression. This model is particularly valuable in cancer research, where AURKB is often overexpressed and linked to tumorigenesis, making it a potential target for therapeutic interventions. |
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Organism | Human |
Tissue | Endocervix |
Disease | Adenocarcinoma |
Characteristics
Age | 30 years |
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Gender | Female |
Ethnicity | African American |
Morphology | Epithelial-like cells with mosaic stone shape |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HK-ZFN-AURKB-mEGFP (Cytion catalog number 300173) |
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Biosafety level | 1 |
Depositor | Dr. J. Ellenberg, EMBL Heidelberg |
Expression / Mutation
Products | EGFP (Enhanced Green Fluorescent Protein) |
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Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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