PurKine His Tagged Protein Purification Ni-NTA Resin
Form/Appearance
Liquid solution with 50% slurry in 20% ethanol.
Hazard Information
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Storage
Stable for one year at 2-8°C from date of shipment. Do not freeze.
Buffer/Preservatives
Liquid in PBS buffer containing 20% ethanol.
Alternative names
Ni-NTA; His resin; Ni-NTA resin; His tag resin
Note
For research use only
Application notes
PurKine His-Tag Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.