Reagents and instruments for immunology, cell biology and molecular biology.

Hexokinase (HK) Activity Assay Kit

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Cat# E-BC-K610-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle	HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD⁺ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.
Synonyms	HK
Sample Type	Animal tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (440-450 nm, optimum wavelength: 450 nm)
Research Area	Glycolysis And Lipid Metabolism , Energy Metabolism
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	5.93 U/L
Detection Range	5.93-40.11 U/L
Precision	inter-assay CV: 9.1% \| intra-assay CV: 3.3%
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K011-M	Glucose-6-phosphate (G6P) Colorimetric Assay Kit	96T
E-BC-K056-M	Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit	96T
E-BC-K234-M	Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)	500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse spleen tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Mouse heart tissue homogenate	2-6
10% Mouse brain tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat liver tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.