Goat anti-FOXL2 / BPES
Cat# EB06022
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB06022 | Goat Anti-FOXL2 / BPES Antibody | Pep-ELISA, WB, IHC | 100µg specific antibody in 200µl | Human, Mouse |
Target Protein
Principal Names: FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3Official Symbol: FOXL2
Accession Number(s): NP_075555.1
Human GeneID(s): 668
Non-Human GeneID(s): 26927 (mouse)
Immunogen
Peptide with sequence C-DSKTGALHSRLDL, from the C Terminus of the protein sequence according to NP_075555.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Approx. 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and Mouse NP_036150.1). This molecular weight is observed by other Sources. Recommended concentration 1-3ug/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Ovary. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Cow
Specific Reference
This antibody (previous batch) has been successfully used in IF on Mouse:
Padua MB, Fox SC, Jiang T, Morse DA, Tevosian SG.
Simultaneous Gene Deletion of Gata4 and Gata6 Leads to Early Disruption of Follicular Development and Germ Cell Loss in the Murine Ovary.
Biol Reprod. 2014 Jun 4.
PMID: 24899573
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol