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> Goat anti-CDX2

Goat anti-CDX2

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Cat# EB07190

Size : 100ug

Brand : Everest Biotech

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Code Name Applications Availability Size Tested species Grade
EB07190 Goat Anti-CDX2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human
EB07190 1
EB07190 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07190 2
EB07190 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Target Protein

Principal Names: CDX2, caudal type homeo box transcription factor 2, HGNC:1806, CDX-3, CDX3
Official Symbol: CDX2
Accession Number(s): NP_001256.1
Human GeneID(s): 1045
Non-Human GeneID(s): 12591 (mouse), 66019 (rat)
Important Comments: No cross-reactivity expected with CDX1 and 4

Immunogen

Peptide with sequence EQLSPGGQRRNLCE, from the internal region of the protein sequence according to NP_001256.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Colon. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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