Reagents and instruments for immunology, cell biology and molecular biology.

GLUT-1 Polyclonal Antibody

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Cat# E-AB-31556-20

Size : 20uL

Brand : Elabscience

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Verified Samples	Verified Samples in WB: K562 Verified Samples in IHC: Rat kidney Verified Samples in IF: Rat lung
Dilution	WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200
Isotype	IgG
Host	Rabbit
Reactivity	Human, Mouse, Rat
Applications	WB, IHC-p, IF
Clonality	Polyclonal
Immunogen	Synthesized peptide derived from the C-terminal region of human Glut1
Abbre	Glut1
Synonyms	CSE, Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity), DYT17, DYT18, DYT9, EIG12, Erythrocyte/hepatoma glucose transporter, Glu, Glucose transporter 1, Glucose transporter type 1, erythrocyte/brain, facilitated glucose transporter member 1
Swissprot	P11166
Calculated MW	54 kDa
Observed MW	55 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Membrane
Concentration	1 mg/mL
Buffer	Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method	Affinity purification
Research Areas	Cancer, Cardiovascular, Metabolism, Signal Transduction
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background	This gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

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