Reagents and instruments for immunology, cell biology and molecular biology.

Gibberellic acid [77-06-5]

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Cat# HY-N1964-1ml

Size : 10mM/1mL

Brand : MedChemExpress

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Description

Gibberellic Acid is named after the fungus Gibberella fujikuroi. Gibberellic Acid regulates processes in plant development and growth, including seed development and germination, stem and root growth, cell division, and flowering time. Gibberellic Acid also improves plant response to growth stress caused by various environmental stresses, such as cold stress, drought stress, heavy metal stress, etc. Gibberellic Acid also causes increased lipid peroxidation and fluctuations in the antioxidant defense system in rats^[1]^[2]^[3]^[4].

Cellular Effect

Cell Line	Type	Value	Description	References
CA46	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human CA46 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human CA46 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]
HCC1599	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human HCC1599 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HCC1599 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]
HEK-293T	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human HEK293T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HEK293T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]
Hs-578T	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human Hs-578T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human Hs-578T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]
NCI-H446	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]
PSN1	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human PSN1 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human PSN1 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]
RCK8	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human RC-K8 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human RC-K8 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]
SUD4	IC₅₀	> 10000 nM Compound: JRA-001	Cytotoxicity against human SU-DHL-4 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human SU-DHL-4 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 33062173]

In Vitro

Gibberellic acid rescues chickpea seedlings from cold stress. Gibberellic acid (10-20 μM) increases final emergence probability (FEP), helping seeds maintain higher relative water content and lower electrolyte leakage^[2].
Gibberellic acid (0.025 μM) treatment of Echinacea purpurea L. hairy roots increased PAL activity, cell viability, and free radical scavenging activity in hairy root cultures. Gibberellic acid optimizes the production of secondary metabolites in Echinacea purpurea L. hairy root cultures and is beneficial to the growth of hairy roots^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (288.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8871 mL	14.4354 mL	28.8709 mL
5 mM	0.5774 mL	2.8871 mL	5.7742 mL
10 mM	0.2887 mL	1.4435 mL	2.8871 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Purity & Documentation

Purity: ≥98.0%

Data Sheet (278 KB) SDS (393 KB)

COA (253 KB) HNMR (255 KB)

Handling Instructions (2659 KB)

References

[1]. Kwon CT, et al. Gibberellic Acid: A Key Phytohormone for Spikelet Fertility in Rice Grain Production. Int J Mol Sci. 2016 May 23;17(5). [Content Brief]

[2]. Aziz T, et al. Seed priming with gibberellic acid rescues chickpea (Cicer arietinum L.) from chilling stress[J]. Acta Physiologiae Plantarum, 2020, 42: 1-10.

[3]. Celik I, et al. Abcisic acid and gibberellic acid cause increased lipid peroxidation and fluctuated antioxidant defense systems of various tissues in rats. J Hazard Mater. 2007 Sep 30;148(3):623-9. [Content Brief]

[4]. Abbasi BH, et al. Gibberellic acid increases secondary metabolite production in Echinacea purpurea hairy roots. Appl Biochem Biotechnol. 2012 Dec;168(7):2057-66. [Content Brief]

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Gibberellic acid [77-06-5]

Cat# HY-N1964-1ml

Brand : MedChemExpress

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