DAPI (dihydrochloride) [28718-90-3]
Cat# HY-D0814-10mg
Size : 10mg
Brand : MedChemExpress
DAPI dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells.
For research use only. We do not sell to patients.
DAPI dihydrochloride Chemical Structure
CAS No. : 28718-90-3
This product is a controlled substance and not for sale in your territory.
Based on 52 publication(s) in Google Scholar
Other Forms of DAPI dihydrochloride:
- DAPI dilactate In-stock
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DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Pharmaceuticals. 2022 Jun 6;15(6):721.
- The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.
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DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123. [Abstract]
- Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.
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DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952. [Abstract]
- Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.
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DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15;96:468-479. [Abstract]
- CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.
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DAPI dihydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar;68:153146. [Abstract]
- MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.
Description |
DAPI dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1]. |
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In Vitro |
General Protocol MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
350.25 |
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Formula |
C16H17Cl2N5 |
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CAS No. |
28718-90-3 |
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Appearance |
Solid |
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Color |
Light yellow to green yellow |
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Emission (Em) |
451 |
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Excitation (Ex) |
356 |
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SMILES |
N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.06Cl.06Cl |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
-20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvent & Solubility |
In Vitro:
DMSO : 75 mg/mL (214.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 5.56 mg/mL (15.87 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.27% |
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Dyeing Example |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 2.8551 mL | 14.2755 mL | 28.5510 mL | 71.3776 mL |
5 mM | 0.5710 mL | 2.8551 mL | 5.7102 mL | 14.2755 mL | |
10 mM | 0.2855 mL | 1.4276 mL | 2.8551 mL | 7.1378 mL | |
15 mM | 0.1903 mL | 0.9517 mL | 1.9034 mL | 4.7585 mL | |
DMSO | 20 mM | 0.1428 mL | 0.7138 mL | 1.4276 mL | 3.5689 mL |
25 mM | 0.1142 mL | 0.5710 mL | 1.1420 mL | 2.8551 mL | |
30 mM | 0.0952 mL | 0.4759 mL | 0.9517 mL | 2.3793 mL | |
40 mM | 0.0714 mL | 0.3569 mL | 0.7138 mL | 1.7844 mL | |
50 mM | 0.0571 mL | 0.2855 mL | 0.5710 mL | 1.4276 mL | |
60 mM | 0.0476 mL | 0.2379 mL | 0.4759 mL | 1.1896 mL | |
80 mM | 0.0357 mL | 0.1784 mL | 0.3569 mL | 0.8922 mL | |
100 mM | 0.0286 mL | 0.1428 mL | 0.2855 mL | 0.7138 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DAPI dihydrochloride Related Classifications
- Others
- Cell Cycle/DNA Damage
- DNA Stain