Cytokeratin 3, K2P (Keratin K3/K76) (PE)

Cat# C9097-34M-PE-100ul

Size : 100ul

Brand : US Biological

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C9097-34M-PE Cytokeratin 3, K2P (Keratin K3/K76) (PE)

Clone Type
Polyclonal
Host
mouse
Source
rabbit
Isotype
IgG1
Grade
Highly Purified
Applications
IHC WB
Crossreactivity
Bo Hu Mo Rb
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. The cytokeratins are expressed in combinations which characterize the type of epithelium.||Applications: |Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.| |Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2Q1040(AE5)|Host: mouse|Source: rabbit|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).|Purity: Purified by ion exchange chromatography.|Immunogen: Rabbit corneal epithelial keratin|Specificity: Recognizes the 64kD polypeptide (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. |Species Crossreactivity: human, bovine, mouse||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rabbit corneal epithelial keratin
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by ion exchange chromatography.
Specificity
Recognizes the 64kD polypeptide (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. Species Crossreactivity: human, bovine and mouse
References
1. Mikaelian, I., et al., Toxicology Pathology 32: 181-191 (2004). 2. Wang, D., et al., Invest. Ophthalmol. Vis. Sci. 44: 4698-4704 (2003). 3. Schermer, A., et al., J. Cell Biol. 103: 49-62 (1986). 4. Cooper, D., et al., Lab. Invest. 52: 243-256 (1985). 5. Moll, R., et al., Cell 31: 11-24 (1982). 6. Sun, T.T. and Green, H., Cell 14: 469-476 (1978).