Chemiluminescent Western Blot Kit
Cat# orb342274
Size : 1Kit
Brand : Biorbyt
Flow Diagram for Chemiluminescent Western Blot Procedure.
Western Blot. Anti-DYKDDDDK (FLAG®) Chemiluminescent Kit for Western blot was used to detect known amounts of bacterial alkaline phosphatase (BAP) protein containing the FLAG® epitope tag. N-terminal linked and C-terminal linked BAP were diluted into a HeLa whole cell lysates. After separation by SDS-PAGE lysates were transferred to a membrane which was blocked with 5% BLOTTO (p/n orb348624) in TTBS for 1 h at 4 °C. The blot was probed with Anti-DYKDDDDK (FLAG®) followed by HRP anti-Mouse IgG (p/n orb347385) at 1:10000 dilution in blocking buffer for 1 h at 4°C.