Reagents and instruments for immunology, cell biology and molecular biology.

CD8 (PE)

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Cat# 214739-100T

Size : 100Tests

Brand : US Biological

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214739 CD8 (PE)

Clone Type

Polyclonal

Host

mouse

Source

equine

Isotype

IgG1

Grade

Affinity Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

CD8 cell surface antigen which is expressed by a subset of T lymphocytes.||A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that CD8 recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007).||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Hybridoma: |X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: CVS8|Host: mouse|Source: equine|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Equine PBMCs|Specificity: Recognizes equine CD8. Species Crossreactivity: Ass ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Equine PBMCs

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes equine CD8. Species Crossreactivity: Ass

References

1. Lunn, D.P et al (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets Immunology 74: 251-257. 2. Jacks, S. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction. Clin Vaccine Immunol. 14: 669-77. 3. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7. 4. Lunn, D.P. et al. (1998) Report of the second equine leucocyte antigen workshop, Squaw Valley, California July 1995. Vet Immunol Immunopathol. 62: 101-143. 5. Merant, C. et al. (2003) Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry. Vet Res. 34: 791-801. 6. Ibrahim, S (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28 Chapter 5 in PhD Thesis Freie Universität Berlin