CD28 IgG2a Fc, Fusion Protein (Biotin)

Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include, prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60kD and 80kD, respectively.||Molecular Structure: A soluble fusion protein consisting of the extracellular (134 aa) domain of  human CD28 fused to murine IgG2a Fc (232 aa), with a predicted monomeric molecular weight of 41.8kD.||Transfectant Cell Line: CHO||Human CD28-muIg/Biotin Fusion Protein||Functional Application: CD28-muIg fusion protein blocks binding of anti-human CD28 to human tumor cells.||Applications: |Suitable for use in ELISA and Antibody Blocking. Other applications not tested.||Recommended Dilution|ELISA: 0.5ng/ml detects the fusion protein using an anti-CD28 antibody coated capture plate and Streptavidin/HRP as a detector.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.