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> Blue Tetrazolium [1871-22-3]

Blue Tetrazolium [1871-22-3]

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Cat# HY-D0007

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Brand : MedChemExpress


Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF). Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH).

For research use only. We do not sell to patients.

Blue Tetrazolium Chemical Structure

Blue Tetrazolium Chemical Structure

CAS No. : 1871-22-3

This product is a controlled substance and not for sale in your territory.

Description

Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF). Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH)[1].

Molecular Weight

727.64

Formula

C40H32Cl2N8O2
CAS No.

1871-22-3
SMILES

COC(C=C(C1=CC(OC)=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)C=C1)C=C5)=C5N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References

  • [1]. Kregiel D, et al. Succinate dehydrogenase activity assay in situ with blue tetrazolium salt in crabtree-positive Saccharomyces cerevisiae strain[J]. Food Technology and Biotechnology, 2008, 46(4): 376-380.

  • [1]. Kregiel D, et al. Succinate dehydrogenase activity assay in situ with blue tetrazolium salt in crabtree-positive Saccharomyces cerevisiae strain[J]. Food Technology and Biotechnology, 2008, 46(4): 376-380.

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Blue Tetrazolium Related Classifications

  • Others
  • Others
  • Fluorescent Dye

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Blue Tetrazolium1871-22-3Tetrazolium blueFluorescent DyeInhibitorinhibitorinhibit