Reagents and instruments for immunology, cell biology and molecular biology.

Anti-IDH3A antibody (28-366)

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Cat# NB-22-37702-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

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Immunofluorescence analysis of U-2 OS cells using IDH3A Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using IDH3A Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using IDH3A Rabbit polyclonal antibody at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of IDH3A in paraffin-embedded mouse kidney using IDH3A Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of IDH3A in paraffin-embedded human colon carcinoma using IDH3A Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of IDH3A in paraffin-embedded human thyroid cancer using IDH3A Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of IDH3A in paraffin-embedded rat kidney using IDH3A Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Western blot analysis of various lysates using IDH3A Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-IDH3A (28-366) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	IDH3A
Gene ID:	3419
Uniprot ID:	IDH3A_HUMAN
Immunogen Region:	28-366
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_005521.1).
Immunogen Sequence:	TGGVQTVTLIPGDGIGPEIS AAVMKIFDAAKAPIQWEERN VTAIQGPGGKWMIPSEAKES MDKNKMGLKGPLKTPIAAGH PSMNLLLRKTFDLYANVRPC VSIEGYKTPYTDVNIVTIRE NTEGEYSGIEHVIVDGVVQS IKLITEGASKRIAEFAFEYA RNNHRSNVTAVHKANIMRMS DGLFLQKCREVAESCKDIKF NEMYLDTVCLNMVQDPSQFD VLVMPNLYGDILSDLCAGL

Description

Function	Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
Protein Name	Isocitrate Dehydrogenase Nad Subunit Alpha - Mitochondrial Isocitric Dehydrogenase Subunit Alpha Nad(+-Specific Icdh Subunit Alpha
Database Links	Reactome: R-HSA-71403
Cellular Localisation	Mitochondrion
Alternative Antibody Names	Anti-Isocitrate Dehydrogenase Nad Subunit Alpha - Mitochondrial antibody Anti-Isocitric Dehydrogenase Subunit Alpha antibody Anti-Nad(+-Specific Icdh Subunit Alpha antibody Anti-IDH3A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance