Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Histone H3 antibody (Clone: 1G1)

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Cat# NB-22-6938-50

Size : 50ug

Brand : Neo Biotech

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Datasheet (EN)

IF analysis of Hela, diluted at 1:200.
1) Input: Hela Cell Lysate , 2) IP product: IP dilute 1:200
Immunofluorescence analysis of Rat-liver tissue. 1, Histone H3 monoclonal antibody (1G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of Human-liver-cancer tissue. 1, Histone H3 monoclonal antibody (1G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1, Histone H3 monoclonal antibody (1G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, Histone H3 monoclonal antibody (1G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, Histone H3 monoclonal antibody (1G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H3 monoclonal antibody (1G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.
Immunofluorescence analysis of Hela cell. 1, Amyloid-Beta Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Mouse
Applications:	WB/IHC/IF/IP
Reactivity:	Human/Mouse/Rat/Yeast/A.thaliana
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Histone H3.1 and Histone H3.2 and Histone H3.3 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.

Clonality:	Monoclonal
Clone ID:	1G1
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range:	WB 1:2000-5000 IF 1:100-500 IP 1:200 IHC 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	H3C1.H3C2.H3C3.H3C4.H3C6.H3C7.H3C8.H3C10.H3C11.H3C12 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 3020/3021
Uniprot ID:	H31_HUMAN H33_HUMAN H32_HUMAN <
Specificity:	The antibody detects endogenous Histone H3 protein.
Immunogen:	Recombinant Protein of Histone H3

Description

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name	Histone H3.1 Histone H3/A Histone H3/B Histone H3/C Histone H3/D Histone H3/F Histone H3/H Histone H3/I Histone H3/J Histone H3/K Histone H3/L
Database Links	Reactome: R-HSA-1266695 Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-3214815 Reactome: R-HSA-3214841 Reactome: R-HSA-3214842 Reactome: R-HSA-3214847 Reactome: R-HSA-3214858 Reactome: R-HSA-3247509 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379 Reactome: R-HSA-9616222 Reactome: R-HSA-977225 Reactome: R-HSA-983231
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H3.1 antibody Anti-Histone H3/A antibody Anti-Histone H3/B antibody Anti-Histone H3/C antibody Anti-Histone H3/D antibody Anti-Histone H3/F antibody Anti-Histone H3/H antibody Anti-Histone H3/I antibody Anti-Histone H3/J antibody Anti-Histone H3/K antibody Anti-Histone H3/L antibody Anti-H3C1 antibody Anti-H3FA antibody Anti-HIST1H3A.H3C2 antibody Anti-H3FL antibody Anti-HIST1H3B.H3C3 antibody Anti-H3FC antibody Anti-HIST1H3C.H3C4 antibody Anti-H3FB antibody Anti-HIST1H3D.H3C6 antibody Anti-H3FD antibody Anti-HIST1H3E.H3C7 antibody Anti-H3FI antibody Anti-HIST1H3F.H3C8 antibody Anti-H3FH antibody Anti-HIST1H3G.H3C10 antibody Anti-H3FK antibody Anti-HIST1H3H.H3C11 antibody Anti-H3FF antibody Anti-HIST1H3I.H3C12 antibody Anti-H3FJ antibody Anti-HIST1H3J antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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