Synthetic 18 amino acid peptide from internal region of human USP4. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig (100%); Hamster, Opossum (94%); Mouse, Rat, Turkey, Platypus (89%); Chicken, Xenopus (83%).
Concentration
0.7 mg/ml
Dilution range
IHC, IHC-P (5 µg/ml)
Conjugation
Unconjugated
Target
USP4
Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives
PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide
Alternative names
USP4, Deubiquitinating enzyme 4, Unph, Ubiquitin t
Note
For research use only
Application notes
Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.
