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> Tween(R) 20

Tween(R) 20

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Cat# 37470.01

Size : 500G

Brand : SERVA Electrophoresis


(Polysorbate 20, Polyoxyethylene sorbitan monolaureate)
Mr ca. 1200 • CAS [9005-64-5]

Tween® 20, belonging to the family of polysorbates, is a non-ionic surfactant, which effectively prevents non-specific reactions between antibodies, antigens and other molecules (1, 2). The detergent is used in sample preparation and signal transduction research for solubilisation of membrane components (3) and in density centrifugation of viruses (4). Tween® 20 serves as an emulsifier for the preparation of stable oil-in-water emulsions.

Used as a component of:
  • Wash buffer and blocking buffer in Western blot analyses
  • Blocking buffer for immunohistochemistry
  • Wash buffer for ELISA (Enzyme-linked Immunosorbent Assay)


    Free dioxane
    Free ethylene oxide
    n
    Non animal origin

    		 max. 1 ppm
    max. 1 ppm
    ca. 20, CMC (25 °C) 0.059 mM, HLB 16.7
    Tween® = registered trademark of CRODA International PLC


    References:
    1. Thean, E.T. & Toh, B.H. (1989) Anal. Biochem. 177, 256-8
    2. Tovey, E.R. et al. (1989) Electrophoresis 10, 243-9
    3. Lund, S. et al. (1989) J. Biol. Chem. 264, 4907-15
    4. Boeye, A. & DeRees, A. (1989) Arch. Virol. 107, 77-84

    EINECS: 500-018-3 • WGK: 1L • HS: 34024200
    Storage Temperature: +15 °C to +30 °C

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