Trypan Blue 0.4% Solution
Cat# 25561-250
Size : 250ml
Brand : Polysciences
Trypan Blue 0.4% Solution
Catalog Number | Unit Size | QTY | |
---|---|---|---|
25561-250 | 250 mL | | |
Product Overview
The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply mixed with dye and then visually examined to determine whether cells take up or exclude dye. In the protocol presented here, a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm.
This product is for research use only and is not intended for use in humans or for in vitro diagnostic use.
Shipping Requirements:
None
References
Strober, W. 1997. Current Protocols in Cytometry. A.3B.1-A.3B.2.
Safety & Storage
Hazards:
Sodium azide may react with lead and copper plumbing to form explosive metal azides. Upon disposal of material, flush with a large volume of water to prevent azide accumulation. Please consult the Safety Data Sheet for more information.
Handling:
Containers which are opened must be carefully resealed and kept upright to prevent leakage.
Storage:
Store at room temperature