TransTaq®-T DNA Polymerase
Cat# AP122-01
Size : 250units
Brand : TransGen Biotech
ManualTransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.
• TransTaq®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent“A”can be generated at the 3’end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.
Applications
• Complex templates
• TA cloning
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP122-01/11 | AP122-02/12 | AP122-03/13 |
| TransTaq®-T DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 10×TransTaq® | 1.2 ml ×1 | 1.2 ml ×1 | 1.2 ml ×6 |
| 2.5 mM dNTPs | - / 400 μl ×1 | - / 800 μl ×1 | - / 800 μl ×6 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml ×1 | 1 ml ×2 |
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