TransTaq® DNA Polymerase High Fidelity (HiFi)
Cat# AP131-03
Size : 6×500units
Brand : TransGen Biotech
ManualTransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
• TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.
Applications
• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP131-01/11 | AP131-02/12 | AP131-03/13 |
| TransTaq® HiFi DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 10×TransTaq® HiFi Buffer I | 1.2 ml×1 | 1.2 ml×1 | 1.2 ml×6 |
| 10×TransTaq® HiFi Buffer II | 1.2 ml×1 | 1.2 ml×1 | 1.2 ml×6 |
| 2.5 mM dNTP | -/400 μl×1 | -/800 μl×1 | -/800 μl×6 |
| 10×GC Enhancer | 200 μl×1 | 400 μl×1 | 1 ml×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml×1 | 1 ml×2 |
Li P, Du J, Goodier J L, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic acids research, Nucleic Acids Res. 2017, 45(8):4619-4631.
Che L H, Zhang SQ, Li Y, et al. Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep and shallow-level divergences[J]. Molecular Ecology Resources, 2017, 17(6):1342.
You CX , Zhao Q , Wang XF , et al. A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture in apple[J]. Plant Biotechnology Journal, 2014, 12(2):183-192.
Gu P , Yang F , Kang J , et al. One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli[J]. Microbial Cell Factories, 2012, 11(1):30.
Wang G H , Sun B F , Xiong T L , et al. Bacteriophage WO Can Mediate Horizontal Gene Transfer in Endosymbiotic Wolbachia Genomes[J]. Frontiers in Microbiology, 2016, 7:1867.