TransStart® Top Green qPCR SuperMix (+Dye I )
Cat# AQ132-13
Size : 15×1ml
Brand : TransGen Biotech
ManualTransStart® Top Green qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except primer and template. It contains TransStart® TopTaq DNA Polymerase, SYBR Green I, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.
• TransStart® TopTaq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃)
| Component | AQ131-01 | AQ131-02 | AQ131-03 | AQ131-04 |
| 2×TransStart® Top Green qPCR SuperMix | 1 ml | 5×1 ml | 15×1 ml | 25×1 ml |
| Passive Reference Dye (50×) | 40 μl | 200 μl | 600 μl | 1 ml |
| Nuclease-free Water | 1 ml | 5 ml | 3×5 ml | 5×5 ml |
Si W , Huang W , Zheng Y , et al. Dysfunction of the Reciprocal Feedback Loop between GATA3- and ZEB2-Nucleated Repression Programs Contributes to Breast Cancer Metastasis[J]. Cancer Cell, 2015, 27(6):822-836.
Wei Y , Hu W , Wang Q , et al. Identification, transcriptional and functional analysis of heat-shock protein 90s in banana (Musa acuminata L.) highlight their novel role in melatonin-mediated plant response to Fusarium wilt[J]. Journal of Pineal Research, 2017, 62(1):e12367.
Li P, Du J, Goodier JL, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic Acids Research, 2017, 45(8):4619-4631.
Sun X, Wang C, Xiang N, et al. Activation of secondary cell wall biosynthesis by miR319-targeted TCP4 transcription factor[J]. Plant Biotechnol . 2017, 15(10):1284-1294.
Ju Z , Cao D , Gao C , et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.