TransStart® FastPfu DNA Polymerase (with 2.5 mM dNTPs)
Cat# AP221-11
Size : 250units
Brand : TransGen Biotech
ManualTransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
• Extension rate is about 2-4 kb/min.
• TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP221-01/11 | AP221-02/12 | AP221-03/13 |
| TransStart® FastPfu DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 5×TransStart® FastPfu Buffer | 1.2 ml×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | - / 500 μl×1 | - / 1 ml ×1 | - / 1 ml ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
| PCR Stimulant | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 200 μl×1 | 1 ml ×1 | 1 ml ×2 |
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