TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix
Cat# AT311-02
Size : 50rxns×20ulsystem
Brand : TransGen Biotech
ManualUnique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous
genomic DNA removal and cDNA synthesis. After cDNA synthesis, TranScript® RT/RI and gDNA remover are inactivated by
heating at 85°C for 5 seconds.
Features
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol.
• Easy to operate.
• cDNA up to 12 kb.
Applications
Multiple copy and low copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AT311-02 (50 rxns) | AT311-03 (100 rxns) | AT311-04 (500 rxns) |
| TransScript® RT/RI Enzyme Mix | 50 μl | 100 μl | 5×100 μl |
| gDNA Remover | 50 μl | 100 μl | 5×100 μl |
| 2×TS Reaction Mix | 500 μl | 1 ml | 5×1 ml |
| Random Primer(N9) (0.1 μg/μl) | 50 μl | 100 μl | 5×100 μl |
| Anchored Oligo(dT)18 Primer (0.5 μg/μl) | 50 μl | 100 μl | 5×100 μl |
| RNase-free Water | 500 μl | 1 ml | 5 ml |
Chen J , Ou Y , Yang Y , et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018,557(7706):585-589.
Yang Y , Liu B , Xu J , et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency[J]. Cell, 2017, 169(2):243-257.e25.
Wu D , Qi T , Li W X , et al. Viral effector protein manipulates host hormone signaling to attract insect vectors[J]. Cell Research, 2017, 27(3):402-415.
Shao X , Liu Y , Yu Q , et al. Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b[J]. Cell Research, 2016,26(5):593-612.
Ma D , Hu Y , Yang C , et al. Genetic basis for glandular trichome formation in cotton[J]. Nature Communications, 2016, 7:10456.