TransScript® Green miRNA Two-Step qRT-PCR SuperMix
Cat# AQ202-01
Size : RTsystem/qPCRsystem: 20rxns×20μl/500rxns×20μl
Brand : TransGen Biotech
ManualTransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStartTM Green qPCR SuperMix is used for miRNA quantification.
Highlights
• Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA.
• One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube.
• High amplification efficiency, specificity and sensitivity ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).
Applications
Multiple copy and low copy gene detection.
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃)
| Component | AQ202-01 |
| TransScript® miRNA RT Enzyme Mix | 20 μl |
| 2×TS miRNA Reaction Mix | 200 μl |
| Universal miRNA qPCR Primer (10 μM) | 200 μl |
| 2×PerfectStartTM Green qPCR SuperMix | 5×1 ml |
| Passive Reference Dye (50×) | 200 μl |
| RNase-free Water | 1 ml |
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