| Component | AT301-02 | AT301-03 |
| TransScript® RT/RI Enzyme Mix | 50 μl | 100 μl |
| 2×TS Reaction Mix | 500 μl | 1 ml |
| Random Primer (N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo (dT)18 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
TransScript® First-Strand cDNA Synthesis SuperMix
Cat# AT301-02
Size : 50rxns×20ul
Brand : TransGen Biotech
TransScript® First-Strand cDNA Synthesis SuperMix
Catalog Number： AT301-02
Price：Please inquire first
Specification：
50 rxns×20 µl system
100 rxns×20 µl system
Product Details
TransScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript RT/RI Enzyme Mix and 2xTS Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.
Applications
Multiple copy and low copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Composition
References
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3 Wang Y, Gong Q, Wu Y, et al. A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants[J]. Cell Host & Microbe, 2021.(IF 21.02)
4 Yan Z, Wang D, An C, et al. The antimicrobial peptide YD attenuates inflammation via miR-155 targeting CASP12 during liver fibrosis[J]. Acta pharmaceutica Sinica B, 2021.(IF 11.41)
5 Chen J, He Y, Liang H, et al. Regulation of PDGFR-β gene expression by targeting the G-vacancy bearing G-quadruplex in promoter[J]. Nucleic Acids Research, 2021.(IF 16.90)
6 Huang G, Wu Z, Percy R G, et al. Genome sequence of Gossypium herbaceum and genome updates of Gossypium arboreum and Gossypium hirsutum provide insights into cotton A-genome evolution[J]. Nature genetics, 2020.(IF 27.00)
7 Chen G, Wang D, Wu B, et al. Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation[J]. Nature communications, 2020.(IF 12.12)
8 Xiang C, Du Y, Meng G, et al. Long-term functional maintenance of primary human hepatocytes in vitro[J]. Science, 2019.(IF 41.00)
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