TransFast® Taq DNA Polymerase
Cat# AP101-01
Size : 500units
Brand : TransGen Biotech
TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.
• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.
Applications
• Routine PCR
• High throughput PCR
• Colony PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Component | AP101-01/11 | AP101-02/12 |
TransFast® Taq DNA Polymerase | 500 U×1 | 500 U×6 |
10×TransFast® Taq Buffer | 1.2 ml ×1 | 1.2 ml ×6 |
2.5 mM dNTPs | - / 800 μl ×1 | - / 800 μl ×6 |
6×DNA Loading Buffer | 1 ml×1 | 1 ml ×2 |
Cao Y X , Xiao W H , Liu D , et al. Biosynthesis of odd-chain fatty alcohols in Escherichia coli[J]. Metabolic Engineering, 2015, 29:113-123.
Wang Y , Hou Y , Gu H , et al. The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis[J]. The Plant Journal, 2012, 69(2):227-240.
Zhang Y , Ji G , Wang R . Drivers of nitrous oxide accumulation in denitrification biofilters with low carbon:nitrogen ratios[J]. Water Research, 2016,106:79-85.
Zhu X J , Sun S , Xie B , et al. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769.
Xia W, Lu H, Xia M, et al. A Novel Glycoside Hydrolase Family 113 Endo-β-1,4-Mannanase from Alicyclobacillus sp. Strain A4 and Insight into the Substrate Recognition and Catalytic Mechanism of This Family.[J]. Applied & Environmental Microbiology, 2016, 82(9):2718.