TransDirect® Plant Tissue PCR Kit
Cat# AD301-02
Size : 500rxns×20ulsystem
Brand : TransGen Biotech
ManualTransDirect® Plant Tissue PCR Kit uses a unique lysis buffer to lyse plant tissues (fresh or frozen). The resulting lysate without purification can be directly used as PCR template. 2×TransDirect® PCR SuperMix (+dye) is highly resistant to various PCR inhibitors present in plant tissues. PCR product can be directly used for gel electrophoresis.
• No DNA extraction needed.
• Ready-to-use SuperMix.
• DNA fragment up to 2 kb.
• For plants with moderate contents of polysaccharides or polyphenols.
Applications
• Direct amplification from unpurified lysate. Suitable for high throughput applications.
• Amplification of genomic DNA fragment up to 2 kb.
Storage
2×TransDirect® PCR SuperMix (+dye) at -20°C for two years, others at room temperature (15-25°C) for two years.
Shipping
2×TransDirect® PCR SuperMix (+dye) on dry ice (-70 ℃); others at room temperature.
| Component | AD301-01 | AD301-02 |
| PD1 Buffer | 4 ml | 20 ml |
| PD2 Buffer | 4 ml | 20 ml |
| 2×TransDirect® PCR SuperMix (+dye) | 1 ml | 5×1 ml |
| Nuclease-free Water | 5 ml | 25 ml |