Reagents and instruments for immunology, cell biology and molecular biology.

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

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Cat# E-BC-K019-S-100

Size : 100Assays

Brand : Elabscience

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Detection Principle	The superoxide anion free radical (O^2-) can be produced by xanthine and xanthine oxidase reaction system, O^2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O^2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.
Detection Principle Image
Synonyms	T-SOD
Sample Type	Serum,Plasma,Urine,Cell,Cell culture supernatant,Tissue
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (550 nm)
Research Area	Oxidative Stress , Plant Physiology Series
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period	12 months
Sensitivity	4.7 U/mL
Detection Range	4.7-166 U/mL
Precision	inter-assay CV: 6.3% \| intra-assay CV: 2.8%
Assay Time	1 h 40 min

Cat.No.	Product Name	Sizes
E-BC-K022-S	CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
HepG2 supernatant	1
HepG2 cell	8-10
Mouse serum	3-5
10% Mouse liver tissue homogenate	40-60
10% Rat kidney tissue homogenate	15-20
Human urine	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.