Total Iron Colorimetric Assay Kit
Detection principle
Performance characteristics
Sample type | serum, tissue |
Sensitivity | 0.4 μmol/L |
Detection range | 0.4-50 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 80min |
Precision | Average inter-assay CV: 1.500%Average intra-assay CV: 1.300% |
Storage | 2-8℃ |
Valid period | 12 months |
Images
The increased iron content in 4T1 cells showed significantly difference between different treatments.
H Tian et al developed a targeted nanomodulator that can induced tumor cell ferroptosis. Iron (Fe) level in mouse tumor cells 4T1 were determined using total iron colorimetric assay kit (E-BC-K772-M).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.4-50 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Mouse serum | 1-2 |
Rat serum | 1 |
10% Mouse liver tissue homogenate | 2-3 |
10% Rat lung tissue homogenate | 1 |
10% Mouse heart tissue homogenate | 1 |
10% Rat spleen tissue homogenate | 2-3 |
10% Epipremnum aureum leaf tissue homogenate | 1 |
Note: The diluent is reagent 1.
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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Frontiers in Veterinary Science (2021) IF: 3.412
Deletion of the c2515 and c2516 Genes Affects Iron Uptake and Virulence of APEC O1 Strain E516PMID: 33912608
Sample: Cell Sample,Tissue Homogenate -
ENVIRONMENTAL TOXICOLOGY (2023) IF: 4.109
GPX4 utilization by selenium is required to alleviate cadmium-induced ferroptosis and pyroptosis in sheep kidneyDOI: 10.1002/tox.23740
Sample: Kidney -
BIOLOGY OF REPRODUCTION (2021) IF: 4.285
Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J miceSample: Serum,Ovary -
PHYTOMEDICINE (2022) IF: 6.656
Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stressPMID: 35905566
Sample: Chondrocytes