TMB [54827-17-7]
Cat# HY-15930-500mg
Size : 500mg
Brand : MedChemExpress
TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
For research use only. We do not sell to patients.
TMB Chemical Structure
CAS No. : 54827-17-7
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Other Forms of TMB:
- TMB dihydrochloride In-stock
- TMB monosulfate In-stock
- TMB dihydrochloride x.hydrate Get quote
- TMB dihydrochloride hydrate Get quote
Description |
TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2]. |
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In Vitro |
TMB can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
240.34 |
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Formula |
C16H20N2 |
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CAS No. |
54827-17-7 |
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Appearance |
Solid |
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Color |
White to light yellow |
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SMILES |
NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Solvent & Solubility |
In Vitro:
DMSO : 25 mg/mL (104.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
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Purity & Documentation |
Purity: 99.95% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 4.1608 mL | 20.8039 mL | 41.6077 mL | 104.0193 mL |
5 mM | 0.8322 mL | 4.1608 mL | 8.3215 mL | 20.8039 mL | |
10 mM | 0.4161 mL | 2.0804 mL | 4.1608 mL | 10.4019 mL | |
15 mM | 0.2774 mL | 1.3869 mL | 2.7738 mL | 6.9346 mL | |
20 mM | 0.2080 mL | 1.0402 mL | 2.0804 mL | 5.2010 mL | |
25 mM | 0.1664 mL | 0.8322 mL | 1.6643 mL | 4.1608 mL | |
30 mM | 0.1387 mL | 0.6935 mL | 1.3869 mL | 3.4673 mL | |
40 mM | 0.1040 mL | 0.5201 mL | 1.0402 mL | 2.6005 mL | |
50 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0804 mL | |
60 mM | 0.0693 mL | 0.3467 mL | 0.6935 mL | 1.7337 mL | |
80 mM | 0.0520 mL | 0.2600 mL | 0.5201 mL | 1.3002 mL | |
100 mM | 0.0416 mL | 0.2080 mL | 0.4161 mL | 1.0402 mL |
TMB Related Classifications
- Others
- Others NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
- Fluorescent Dye Reactive Oxygen Species
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
TMB54827-17-7BM blue Sure Blue TMBFluorescent DyeReactive Oxygen Speciesdyestuffchromogenic agentsinglet oxygenprobeInhibitorinhibitorinhibit