Reagents and instruments for immunology, cell biology and molecular biology.
 
> TMB [54827-17-7]

TMB [54827-17-7]

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Cat# HY-15930-5g

Size : 5g

Brand : MedChemExpress


TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.

For research use only. We do not sell to patients.

TMB Chemical Structure

TMB Chemical Structure

CAS No. : 54827-17-7

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of TMB:

  • TMB dihydrochloride In-stock
  • TMB monosulfate In-stock
  • TMB dihydrochloride x.hydrate Get quote
  • TMB dihydrochloride hydrate Get quote
Description

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].
In Vitro

TMB can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TMB Related Antibodies
Molecular Weight

240.34

Formula

C16H20N2
CAS No.

54827-17-7
Appearance

Solid

Color

White to light yellow

SMILES

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (104.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1608 mL 20.8039 mL 41.6077 mL
5 mM 0.8322 mL 4.1608 mL 8.3215 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.95%

COA (246 KB) HNMR (238 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

  • [1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952.  [Content Brief]

    [2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56.  [Content Brief]

  • [1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952.

    [2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1608 mL 20.8039 mL 41.6077 mL 104.0193 mL
5 mM 0.8322 mL 4.1608 mL 8.3215 mL 20.8039 mL
10 mM 0.4161 mL 2.0804 mL 4.1608 mL 10.4019 mL
15 mM 0.2774 mL 1.3869 mL 2.7738 mL 6.9346 mL
20 mM 0.2080 mL 1.0402 mL 2.0804 mL 5.2010 mL
25 mM 0.1664 mL 0.8322 mL 1.6643 mL 4.1608 mL
30 mM 0.1387 mL 0.6935 mL 1.3869 mL 3.4673 mL
40 mM 0.1040 mL 0.5201 mL 1.0402 mL 2.6005 mL
50 mM 0.0832 mL 0.4161 mL 0.8322 mL 2.0804 mL
60 mM 0.0693 mL 0.3467 mL 0.6935 mL 1.7337 mL
80 mM 0.0520 mL 0.2600 mL 0.5201 mL 1.3002 mL
100 mM 0.0416 mL 0.2080 mL 0.4161 mL 1.0402 mL
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TMB Related Classifications

  • Others
  • Others NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  • Fluorescent Dye Reactive Oxygen Species
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TMB54827-17-7BM blue Sure Blue TMBFluorescent DyeReactive Oxygen Speciesdyestuffchromogenic agentsinglet oxygenprobeInhibitorinhibitorinhibit

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