TJP1 Human siRNA Oligo Duplex (Locus ID 7082)
Cat# SR322042
Size : 1kit
TJP1 Human siRNA Oligo Duplex (Locus ID 7082)
SKU
SR322042
TJP1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
In Stock*
| Product Data | |||||||
| Purity | HPLC purified | ||||||
|---|---|---|---|---|---|---|---|
| Quality Control | Tested by ESI-MS | ||||||
| Sequences | Available with shipment | ||||||
| Sequences | Available with shipment | ||||||
| Components | TJP1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 7082)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml Once you identified the best-performing siRNA sequence of the kit, you can order a single siRNA individually.
If you need negative control, please order SR30004 (2 nmol of scramble siRNA control) | ||||||
| Stability | One year from date of shipment when stored at -20°C. | ||||||
| # of transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). | ||||||
| Note | Single siRNA duplex (10nmol) can be ordered. | ||||||
| Reference Data | |||||||
| RefSeq | NM_001301025, NM_001301026, NM_003257, NM_175610, NM_001330239, NM_001355012, NM_001355013, NM_001355014, NM_001355015 | ||||||
| UniProt ID | Q07157 | ||||||
| Synonyms | ZO-1 | ||||||
| Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] | ||||||
| Performance Guaranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at tech@biotrend-usa.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). | ||||||
Write Your Own Review
| Product Manuals |
| FAQs |
|
| SDS |
| RNAi Resources |
|
Related Products
Check items to add to the cart or
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.