Flow Cytometry: 1 µg/ml Western Blotting. Immunocytochemistry. Functional Assay: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells. Other studies approve that the antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase.
Reactivity
Mouse
Antibody Host
Mouse
Isotype
IgG3
Clonality
Monoclonal
Immunogen
Thymocytes from C3H/Di mice (alelic form: Thy-1.2 positive)
Specificity
The antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of Mouse Thy-1 (CD90). It does not cross-react with Thy-1.1 alloantigen. Thy-1 is a 18 kDa cell surface GPI linked glycoprotein (Immunoglobulin superfamily); in the mouse it is expressed on all thymocytes and peripheral T lymphocytes. Negative Species: Human, Rat.
Buffer
Azide free PBS, pH~7.4, sterilized by filteration State: Azide Free State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: None
CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissueand cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.
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