Tetramethylrhodamine Methyl Ester, Perchlorate[115532-50-8]
Cat# 70017
Size : 25MG
Brand : Biotium
| CAS number | 115532-50-8 |
|---|---|
| Probe cellular localization | Mitochondria |
| For live or fixed cells | For live/intact cells |
| Assay type/options | Real-time imaging |
| Cell permeability | Membrane permeant |
| Apoptosis/viability marker | Mitochondrial potential |
| Potential dependence | Mitochondrial potential-dependent |
| Colors | Red |
| Excitation/Emission | 549/574 nm |
Protocols
Supporting Documents
Product Description
TMRM (Tetramethylrhodamine methyl ester, perchlorate) and TMRE (70016) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation. The dyes do not form aggregates in cell membranes and have minimal interaction with membrane proteins. Thus, the transmembrane distribution of the dyes is directly related to the membrane potential according to the Nernst equation. We also offer TMRE, 2 mM in DMSO.
- λEx/λEm (MeOH) = 548/573 nm
- Red solid soluble in DMSO, DMF or EtOH
- Store at 4°C and protect from light
- C25H25ClN2O7
- MW: 501
- [115532-50-8]
References
1. Meth Cell Biol 38, 195 (1993).
2. Meth Cell Biol 30, 193 (1989).
3. Biophys J 56, 1053 (1989).
4. Biophys J 53, 785 (1988).
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