TBX4 (NM_018488) Human Tagged ORF Clone
Cat# RC217451
Size : 10ug
| Product Data | |
| Target Symbol | TBX4 |
|---|---|
| Synonyms | ICPPS; PAPPAS; SPS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC217451 representing NM_018488 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAGGATAAGGGCCTGTCCGAGAGCGAGGAGGCCTTCCGGGCCCCGGGCCCAGCGCTCGGAGAGG CCAGCGCAGCCAACGCCCCCGAGCCCGCGCTGGCAGCGCCGGGCCTCAGCGGAGCCGCGCTAGGCAGCCC CCCGGGACCCGGGGCCGACGTCGTCGCCGCCGCCGCCGCGGAGCAGACCATCGAGAACATCAAGGTGGGG CTGCATGAGAAGGAGCTCTGGAAGAAGTTCCACGAGGCGGGCACCGAGATGATCATCACTAAGGCTGGCA GGAGGATGTTCCCCAGCTACAAGGTAAAAGTCACAGGCATGAACCCCAAGACCAAGTATATCCTGCTGAT TGACATTGTCCCTGCCGATGACCATCGCTACAAGTTCTGTGACAACAAATGGATGGTGGCAGGGAAGGCT GAGCCAGCCATGCCAGGAAGGCTGTATGTCCACCCGGATTCTCCTGCCACAGGAGCCCACTGGATGCGGC AGCTGGTCTCCTTCCAGAAGCTGAAGCTGACAAACAACCACCTGGACCCCTTTGGCCATATCATCCTCAA CTCTATGCACAAGTACCAGCCGCGGCTCCACATCGTTAAGGCTGATGAGAACAATGCTTTCGGCTCCAAA AACACTGCTTTCTGCACCCACGTGTTCCCAGAGACCTCCTTCATCTCTGTGACCTCCTACCAGAATCACA AGATCACCCAGCTGAAAATTGAGAACAACCCTTTTGCCAAGGGATTCCGGGGCAGTGATGACAGTGACCT GCGTGTGGCCCGACTGCAGAGCAAAGAATACCCCGTGATTTCCAAAAGCATCATGAGGCAGAGGCTCATC TCCCCCCAGCTCTCAGCCACACCGGACGTGGGCCCCCTGCTCGGCACCCACCAGGCACTCCAGCACTACC AGCACGAGAACGGGGCACACTCACAGCTCGCGGAGCCGCAGGACCTGCCCCTCAGCACCTTTCCCACCCA GAGGGACTCAAGCCTCTTCTATCACTGCCTGAAAAGACGAGACGGTACCCGCCACCTGGACTTACCTTGC AAGCGATCCTATCTGGAAGCCCCCTCTTCGGTGGGGGAGGATCACTATTTCCGTTCCCCCCCTCCCTACG ACCAGCAAATGCTGAGCCCCTCCTACTGCAGTGAGGTGACCCCCAGAGAAGCATGTATGTACTCAGGTTC AGGGCCCGAGATTGCCGGGGTGTCTGGGGTGGACGACCTGCCCCCACCTCCGCTGAGCTGTAACATGTGG ACTTCAGTGTCGCCGTACACCAGCTATAGCGTGCAGACGATGGAGACTGTGCCGTACCAGCCCTTCCCCA CGCACTTCACCGCCACCACCATGATGCCGCGGCTGCCCACCCTCTCCGCTCAGAGCTCCCAGCCACCAGG AAATGCCCACTTTAGTGTCTACAATCAGCTCTCCCAGTCTCAGGTCCGAGAGCGGGGGCCCAGCGCCTCA TTCCCAAGAGAGCGCGGCCTCCCCCAAGGGTGTGAGAGGAAGCCACCCTCGCCACATCTAAATGCTGCCA ATGAGTTTCTCTACTCTCAAACCTTCTCCTTGTCCCGAGAATCTTCCTTACAGTACCATTCAGGAATGGG GACTGTGGAGAACTGGACTGACGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC217451 representing NM_018488 Red=Cloning site Green=Tags(s) MLQDKGLSESEEAFRAPGPALGEASAANAPEPALAAPGLSGAALGSPPGPGADVVAAAAAEQTIENIKVG LHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKA EPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSK NTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFRGSDDSDLRVARLQSKEYPVISKSIMRQRLI SPQLSATPDVGPLLGTHQALQHYQHENGAHSQLAEPQDLPLSTFPTQRDSSLFYHCLKRRDGTRHLDLPC KRSYLEAPSSVGEDHYFRSPPPYDQQMLSPSYCSEVTPREACMYSGSGPEIAGVSGVDDLPPPPLSCNMW TSVSPYTSYSVQTMETVPYQPFPTHFTATTMMPRLPTLSAQSSQPPGNAHFSVYNQLSQSQVRERGPSAS FPRERGLPQGCERKPPSPHLNAANEFLYSQTFSLSRESSLQYHSGMGTVENWTDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_018488 |
| ORF Size | 1635 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biotrend-usa.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018488.3 |
| RefSeq Size | 2470 bp |
| RefSeq ORF | 1638 bp |
| Locus ID | 9496 |
| UniProt ID | P57082 |
| Cytogenetics | 17q23.2 |
| MW | 60 kDa |
| Summary | This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4, which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene, like TBX2, maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb, but not in forelimb buds, suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq, Jul 2008] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC217451L1 | Lenti ORF clone of Human T-box 4 (TBX4), Myc-DDK-tagged | 10 ug | | |
| RC217451L2 | Lenti ORF clone of Human T-box 4 (TBX4), mGFP tagged | 10 ug | | |
| RC217451L3 | Lenti ORF clone of Human T-box 4 (TBX4), Myc-DDK-tagged | 10 ug | | |
| RC217451L4 | Lenti ORF clone of Human T-box 4 (TBX4), mGFP tagged | 10 ug | | |
| RG217451 | TBX4 (tGFP-tagged) - Human T-box 4 (TBX4) | 10 ug | | |
| SC304597 | TBX4 (untagged)-Human T-box 4 (TBX4) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.